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Restriction Enzymes: Endodeoxyribonucleas, referred to as restriction enzymes, are enzymes that recognize specific nucleotide sequences and cleave phosphodiester bonds between two nucleotides at specific positions in each strand, according to Restriction enzyme structure, cofactor requirements cut and mode of action, restriction enzymes can be divided into three types, namely Type I, Type II and Type III, , Restriction enzymes are an essential tool enzyme in gene cloning and editing.
Name: EcoRI
product manual:
Recognition sites
isoschizomers | compatible ends | single letter code
Activity in different reaction buffers
NEBuffer 1.1: 25%
NEBuffer 2.1: 100 *%
NEBuffer 3.1: 50%
CutSmart Buffer: 50%
characteristic
Recombinant enzyme, time-saving enzyme.
Reaction conditions
EcoRI reaction buffer, 37 ° C.
Heat inactivation: 65 ° C for 20 minutes.
Concentration
20,000 and 100,000 units / ml.
Methylation sensitivity
Sensitive to CpG methylation in mammalian genomic DNA.
* Asterisk activity may appear in this buffer.
Name: EcoRI
product manual:
Recognition sites
isoschizomers | compatible ends | single letter code
Activity in different reaction buffers
NEBuffer 1.1: 25%
NEBuffer 2.1: 100 *%
NEBuffer 3.1: 50%
CutSmart Buffer: 50%
characteristic
Recombinant enzyme, time-saving enzyme.
Reaction conditions
EcoRI reaction buffer, 37 ° C.
Heat inactivation: 65 ° C for 20 minutes.
Concentration
20,000 and 100,000 units / ml.
Methylation sensitivity
Sensitive to CpG methylation in mammalian genomic DNA.
* Asterisk activity may appear in this buffer.
